Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques:

Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Bacterial attachment to and invasion of, and IL-8 production by, HGEC a

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques:

Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Light micrographs of attachment of F. nucleatum 12230 to HGEC. (A) Wild-type bacteria; (B), the lam mutant. The images were taken using a 40× objective. The filaments indicated by the arrows are F. nucleatum.

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques: Bacteria, Mutagenesis

Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Attachment and invasion of KB cells by F. nucleatum a

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques:

Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Effects of metabolic inhibitors on invasion by F. nucleatum 12230

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques:

Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Effects of sugars on attachment of F. nucleatum to KB cells

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques:

Journal:

Article Title: Interactions between Periodontal Bacteria and Human Oral Epithelial Cells: Fusobacterium nucleatum Adheres to and Invades Epithelial Cells

doi:

Figure Lengend Snippet: Transmission electron micrographs of invasion of HGEC and KB by F. nucleatum 12230. (A) F. nucleatum entering HGEC; (B) F. nucleatum internalized in HGEC, with the membrane of the vacuole indicated by the arrow in the inset; (C) F. nucleatum invading and internalized in KB, as indicated by the arrows. C, cytoplasm; N, nucleus. Numbers in parentheses indicate original magnifications.

Article Snippet: F. nucleatum ATCC 10953 and ATCC 25586 were also highly invasive, whereas PK 1594 was only slightly invasive. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Attachment (%) b Invasion (%) b F. nucleatum ATCC 10953 19.2 ± 0.3 7.4 ± 1.1 F. nucleatum ATCC 25586 16.8 ± 0.8 7.3 ± 2.0 F. nucleatum PK 1594 14.4 ± 0.8 0.3 ± 0.0 F. nucleatum 12230 17.7 ± 0.5 4.5 ± 1.9 F. nucleatum 12230 lam 1.5 ± 0.4 0.1 ± 0.0 Open in a separate window a The assays were carried out as described in the text.

Techniques: Transmission Assay, Membrane

Journal: iScience

Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

doi: 10.1016/j.isci.2024.110157

Figure Lengend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

Article Snippet: The bacterial strains used in this study were F. nucleatum subsp. nucleatum ATCC 23726 and ATCC 25586, F. nucleatum subsp. polymorphum ATCC 10953, transtracheal isolate 12230, and tumor isolate F. nucleatum subsp. nucleatum CTI-7 .

Techniques: Binding Assay, Labeling, Incubation, Staining